Analyse the insert DNA

Before you progress, first analyse the insert DNA found below using NEBcutter. This is the same DNA you'll be using in the lab during Practical C. NEBcutter is an online tool used to determine which enzyme sites are present in a DNA sequence. See here for a short video on how to use this tool.

Copy and paste the DNA found here into the NEBcutter tool above.

GAATTCCGGATGAAGCCCACTGTGATGGCGGCCAATCAGGCGCAAAGCAATCCCGCCACCGCCGCCGGTAATAATGTCTCACCCGGCGGTCCCGGTGTCAATATACCCGTAAACCGGGAGTACGTGAGTGGCGGCTACGGATCGCCACAGCAGTCGGCACAGTTCCACACTCCCCAAAACGCTTACGGATCGCCCAGGCAGCAGCAGCAGCACTTCCAGCAGCACCAGCAGGTTCCTCCAAATCAGCAGCAGCAACATTTTCAGCCGACCTTTGGATTCGAACCGAACATGGACATGGACAACATGTTCCCGCGCTCGCACCTGGGCAGGATGCACGATCCCTTCGCCGGCTTCGGCGACTCACGTAAGCGGAGCAGTTTGCACGGTCCCAGTGGTCAAGTCCATGCTGATGATGACTTTCCATCCTATTTCGACGATCCAGACTTTGGTTTCCCCCGCTTCTCAACGATGGGCCGCCGAGGTCGAATGGCCGGTGGGGCCAACACTCACATGGACCACGATGATGACTTCTTTAATCGGCTGCCCTCGGAGTTCCGCCAGTATATCCCAGATGGTTTCGGAGGCAGACGTGGTACCGGAGCTGCTCCTGCACCCGGACAAGTTCCACAGCAGCAACAGCCGCAGCCACAGCCGCAGTACACCCAACAGCAACCCCAGACGCACTTCCAGTTCGGTGTGCCGCCCCAGCAACAGCCAGTCTATGCCGGACCAACCCAGCAGCAGGTGCCGCAGACGCCTTCAAAGCGCCTTTGCGATGCTGCCATCCAGACGGAGGATCCTGCCGGTCGCTCGGAGGTTGATTGTGCCCCGCCGACTAACTTGAACCAGCACGGACTGCGAAACACTGTCGACATGGGCGTGAAGAGTGTCGCCGAGCAGGATCAGGGACTGCGATCCCACTCCGCCCCTCCGCCAGAGCAGCAGCAGCAGAATCTGCAGTATCACCAGCAGCAGCAACAGCATCAGCAACCGGGAGTACATAGCCAACCGTTTGGCACCCAGACAAGTCCGCCCGTTCAGGGTCAGCAGTTCAAGACCTACTATGCGCCGCACCAGCAGACGCATCCCCAGCAAAAGCAGCAGACCCCGCCGCCAGCTCCACAGACCCCGGGTGGCACCTATGTGCGCACCATTCCCATCTTTGTCGAGGGTCGCTCCGAGCCCATCATCAATGCCCACAAGGAGATACCGAACCAGAACGCTCCGCCCAGTGCTCAGGCCCAAGCTCAGGCACAGGCATATGCGCAGGCGCAGCCACAGGCACACGCTGCTCCACAGCAGCACAACAGACCGACGCCACTAAACACCCAGGCGCAACCTGAGCAGCAGGTGCCAGTTGAGGGAGCCGCTGGATTACCGCCACAGACACCACACACCCTCAACTCTATTAACAAAATCCAGGACATTCAGCGCGACGTACTGGAGCTCATGGGCAAGGTGGAGCAGTTCAAGGGAACGCGCGAGGAGAAGGAGTACGCCTACCTGGACGAGATGCTGACCCGCAACCTGCTTAAGCTAGACACCATCGACACAAACGGCAAGGACAGCATTCGTCTGGCCCGAAAGGAGGCTATCAAATGCATTCAGGCTTCGATAAATGTGCTGGAGGCCAAGGCCGAGGAGAATGCCAGAGCGGCATCAGGAGCAGCACCTGCACCTAGCGCAACAGCTCCAGCGGTTGACACAGGTGCTGTTGCTGCCCCAGAAGCGGCAGAACAAAATGCGGAGCCAGTGACTCCACAGCCACAGGATGCTACAAAGATGCAGGAACCCATCCCTCTACCAGCACCACCAAATGCTGCTGCCGTCGAGGGCGCAGCTGCTTCGGAAGCCACCGCCGCGGAGGCGACACAGTCCGCCGTTCAGTCGGAGACAACCACAACGACGTCGGAATGATGAACACAGCGCCTCTACAAGGCGACCGTTTCGAAGCAGCTACGCAAATTAACCAAATTTAAGTGACAACAGGCGGAGGATAGAGAAGAGTTCCGATCCCGACTCATCAACGGACAGCGACAGACTTGATTTTACTGAATTC

After you've analysed the insert DNA, select all the enzymes from the list below that digest the insert. There are 9 correct answers:


Back